In situ immune autoradiographic identification of cells in heart tissues of mice with coxsackievirus B3-induced myocarditis
In adolescent CD-1 male mice inoculated with a myocarditic coxsackievirus B3 (CVB3m) acute focal lesions containing necrotic myocytes, infiltrating mononuclear cells, and fibroblasts develop. With the use of an in situ immune autoradiographic method with rat monoclonal antibodies (MAb) and an /sup 35/S-labeled antibody, viral antigens were detected outside of lesions. Macrophages, T lymphocytes, and natural killer (NK) cells were identified within myocarditic lesions during the acute phase of the disease. Macrophages detected by anti-Mac-1 MAb were in focal areas within myocarditic lesions on Days 4-7 after inoculation. T lymphocytes were detected in myocarditic lesions on Days 4-10, with MAb to Thy-1 and Lyt-1 antigens showing diffuse reaction patterns, suggesting random distribution of these cells in lesions. Focal areas of reactivity were detected with MAbs to L3T4 and Lyt-2 antigens, suggesting clusters of helper and cytotoxic/suppressor T lymphocytes, respectively. NK cells were presumptively detected by asialo GM1 surface marker in lesions at all times. The presence of activated NK cells in lesions was confirmed by assay of mechanically dissociated heart tissues on Day 8. These data describe the temporal sequence and identity of leukocytes entering into CVB3-induced focal myocarditic lesions during the acute phase of disease in CD-1 mice.
- Research Organization:
- Wistar Institute, Philadelphia, PA
- OSTI ID:
- 5669002
- Journal Information:
- Am. J. Pathol.; (United States), Vol. 129:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MYOCARDIUM
INFLAMMATION
VIRAL DISEASES
DIAGNOSIS
AUTORADIOGRAPHY
LYMPHOCYTES
MACROPHAGES
MICE
MONOCLONAL ANTIBODIES
MONOCYTES
SULFUR 35
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
HEART
INFECTIOUS DISEASES
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MAMMALS
MATERIALS
MUSCLES
NUCLEI
ORGANS
PATHOLOGICAL CHANGES
PHAGOCYTES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SULFUR ISOTOPES
SYMPTOMS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics