In situ immune autoradiographic identification of cells in heart tissues of mice with coxsackievirus B3-induced myocarditis

Journal Article · Sun Nov 01 04:00:00 UTC 1987 · Am. J. Pathol.; (United States)

OSTI ID:5669002

Godeny, E K; Gauntt, C J

In adolescent CD-1 male mice inoculated with a myocarditic coxsackievirus B3 (CVB3m) acute focal lesions containing necrotic myocytes, infiltrating mononuclear cells, and fibroblasts develop. With the use of an in situ immune autoradiographic method with rat monoclonal antibodies (MAb) and an /sup 35/S-labeled antibody, viral antigens were detected outside of lesions. Macrophages, T lymphocytes, and natural killer (NK) cells were identified within myocarditic lesions during the acute phase of the disease. Macrophages detected by anti-Mac-1 MAb were in focal areas within myocarditic lesions on Days 4-7 after inoculation. T lymphocytes were detected in myocarditic lesions on Days 4-10, with MAb to Thy-1 and Lyt-1 antigens showing diffuse reaction patterns, suggesting random distribution of these cells in lesions. Focal areas of reactivity were detected with MAbs to L3T4 and Lyt-2 antigens, suggesting clusters of helper and cytotoxic/suppressor T lymphocytes, respectively. NK cells were presumptively detected by asialo GM1 surface marker in lesions at all times. The presence of activated NK cells in lesions was confirmed by assay of mechanically dissociated heart tissues on Day 8. These data describe the temporal sequence and identity of leukocytes entering into CVB3-induced focal myocarditic lesions during the acute phase of disease in CD-1 mice.

• Research Organization: Wistar Institute, Philadelphia, PA
• OSTI ID: 5669002
• Journal Information: Am. J. Pathol.; (United States), Journal Name: Am. J. Pathol.; (United States) Vol. 129:2; ISSN AJPAA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
EVEN-ODD NUCLEI
HEART
INFECTIOUS DISEASES
INFLAMMATION
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MICE
MONOCLONAL ANTIBODIES
MONOCYTES
MUSCLES
MYOCARDIUM
NUCLEI
ORGANS
PATHOLOGICAL CHANGES
PHAGOCYTES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYMPTOMS
VERTEBRATES
VIRAL DISEASES