Enzymatic preparation of. cap alpha. - and. beta. -deuterated or tritiated amino acids with l-methionine. gamma. -lyase

Journal Article · Fri Jan 15 04:00:00 EST 1982 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(82)90586-3· OSTI ID:5658276

Esaki, N; Sawada, S; Tanaka, H; Soda, K

L-Methionine ..gamma..-lyase catalyzes the exchange of ..cap alpha..- and ..beta..-hydrogens of L-methionine and S-methyl-L-cysteine with deuterium or tritium of solvents. The rate of ..cap alpha..-hydrogen exchange with deuterium was about 40 times faster than that of the elimination reactions. The deuterium and tritium were exchanged also with the ..cap alpha..- and ..beta..-hydrogens of the straight-chain amino acids which do not undergo the elimination: L-alanine, L-..cap alpha..-aminobutyrate, L-norvaline, and L-norleucine. No exchange occurs for the D-isomers, acidic L-amino acids, basic L-amino acids, and branched-chain L-amino acids, although ..cap alpha..-hydrogen of glycine, L-trypotophan, and L-phenylalanine is exchanged slowly. These enzymatic hydrogen-exchange reactions facilitate specific labeling of the L-amino acids with deuterium and tritium.

• Research Organization: Kyoto Coll., Japan
• OSTI ID: 5658276
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 119:2; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
CATALYSIS
DEUTERIUM
DRUGS
ELEMENTS
ENZYMES
HYDROGEN
HYDROGEN ISOTOPES
ISOMERS
ISOTOPES
LABELLING
LIGHT NUCLEI
LIPOTROPIC FACTORS
LYASES
METHIONINE
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
STABLE ISOTOPES
SYNTHESIS
TRITIUM
USES
YEARS LIVING RADIOISOTOPES