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Photoinactivation of skin fibroblasts by fractionated treatment with 8-methoxypsoralen and UVA

Journal Article · · J. Invest. Dermatol.; (United States)
;

Guinea pig skin fibroblasts treated with low doses of 8-methoxypsoralen (8-MOP) and long-wave ultraviolet light (UVA) showed a dose-dependent inhibition of /sup 3/H-Thymidine incorporation as determined by liquid scintillation counting. The minimum incubation time necessary to obtain constant inhibition rates was 60 min. By washing the drug was removed from the reactive sites within 30 min. Repeated light exposure at a constant concentration of 8-MOP caused a cumulative inhibition of DNA synthesis. Irradiation of 8-MOP-plus-UVA treated cells, from which the drug was removed, produced a small increase in photoinhibition. Split dose treatment at various time intervals (ranging from 1--48 hr) revealed inhibitory rates, which correspond to the total amount of UVA applied. No recovery effects were seen in cultures treated by single or multiple applications of 8-MOP-plus-UVA.

Research Organization:
Univ. of Kiel, West Germany
OSTI ID:
5654623
Journal Information:
J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 73:2; ISSN JIDEA
Country of Publication:
United States
Language:
English

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Related Subjects

550603 -- Medicine-- External Radiation in Therapy-- (1980-)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CONNECTIVE TISSUE CELLS
COUMARINS
DNA
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GUINEA PIGS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
INHIBITION
MAMMALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PSORALEN
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RIBOSIDES
RODENTS
SKIN
SOMATIC CELLS
SYNTHESIS
THYMIDINE
ULTRAVIOLET RADIATION
VERTEBRATES