Binding of /sup 14/C-5-aminolevulinic acid to a stromal protein from developing pea chloroplasts

Conference · Wed Apr 01 04:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:5653473

Thayer, S S; Castelfranco, P A; Wilkinson, J; Benson, G

/sup 14/-5-Aminolevulinic acid (/sup 14/C-ALA) binds to a stromal protein with an apparent molecular weight of 42-43 KD on LDS and non-denaturing gels. The reaction is rapid. Binding is inhibited by sulfhydryl reagents, mM concentrations of levulinic, dihydroxy heptanoic acids and gabaculine, 10 ..mu..M N-methylprotoporphyrin. Dicarboxilic acids, such as deltaKG, Glu, OAA, do not inhibit. Chloramphenicol, ATP, protoporphyrin, anoxia, light, darkness have no effect. The product, once formed, is stable to treatment with 5% conc. HCl in cold acetone. It can be chased in a second incubation with unlabeled ALA, but not with levulinic acid. No activity was detected in the subplastidic membrane fractions. Western blot analysis failed to reveal any homology between the labeled protein and either cytochrome for ALA dehydratase. This ALA-binding protein was not formed in chloroplasts isolated from fully expanded pea leaves. Therefore, it is deemed likely to participate in ALA metabolism during chloroplast development.

• Research Organization: Univ. of California, Davis
• OSTI ID: 5653473
• Report Number(s): CONF-8707108-
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMINOLEVULINIC ACID
BACTERIA
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL COMPOSITION
CHLOROPLASTS
ELECTROPHORESIS
HYBRIDIZATION
ISOTOPE APPLICATIONS
KETO ACIDS
LABELLED COMPOUNDS
LEAVES
LEGUMINOSAE
LEVULINIC ACID
METABOLISM
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PISUM
PLANTS
PROTEINS
RHIZOBIUM
TRACER TECHNIQUES