Gababuline induces delta-aminolevulinic acid excretion by cyanobacteria

Conference · Mon Mar 31 23:00:00 EST 1986 · Plant Physiol.; (United States)

OSTI ID:5433796

Freeman, L; Guikema, J A

Gabaculine (5-amino-1,3-cyclohexadienylcarboxylic acid) was examined as an inhibitor of Chl biosynthesis in the cyanobacterium, Anacystis nidulans. At 20 ..mu..M, it blocked the synthesis of both Chl and phycocyanin. Similar results were obtained using aminooxyacetic acid. Because gabaculine is well established as an inhibitor of aminotransferase activity, the authors expected it to cause an inhibition of ..delta..-aminolevulinic acid (ALA) synthesis. However, an excretion of ALA was observed instead. Concentrated cell cultures were incubated in the presence of gabaculine, and the spent media was examined for ALA excretion using modified Ehrlick's reagent. Gabaculine induced ALA excretion in normal cultures, and in those stressed by iron or phosphate deficiency. Nitrate deficiency depressed the extent of ALA excretion. These results suggest that, in cyanobacteria, gabaculine inhibits CHl biosynthesis at a site after ALA formation.

• Research Organization: Kansas State Univ., Manhattan
• OSTI ID: 5433796
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 80:4; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550700 -- Microbiology
551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMINOLEVULINIC ACID
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHLOROPHYLL
ENZYME INHIBITORS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KINETICS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOSYNTHETIC BACTERIA
PHYSIOLOGY
PHYTOCHROMES
PIGMENTS
PORPHYRINS
PROTEINS
REACTION KINETICS
SYNTHESIS