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Evolutionarily conserved regions of the human c-myc protein can be uncoupled from transforming activity

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;
The myc family of oncogenes contains coding sequences that have been preserved in different species for over 400 million years. This conservation (which implies functional selection) is broadly represented throughout the C-terminal portion of the human c-myc protein but is largely restricted to three cluster of amino acid sequences in the N-terminal region. The authors have examined the role that the latter three regions of the c-myc protein might play in the transforming function of the c-myc gene. Several mutations, deletions and frameshifts, were introduced into the c-myc gene, and these mutant genes were tested for their ability to collaborate with the EJ-ras oncogene to transform rat embryo fibroblasts. Complete elimination of the first two N-terminal conserved segments abolished transforming activity. In contrast, genes altered in a portion of the second or the entire third conserved segment retained their transforming activity. Thus, the latter two segments are not required for the transformation process, suggesting that they serve another function related only to the normal expression of the c-myc gene.
Research Organization:
Harvard Medical School, Boston, MA
OSTI ID:
5653297
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:1; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
BIOLOGICAL EVOLUTION
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DNA
DNA SEQUENCING
FIBROBLASTS
GENES
MAMMALS
MAN
MOLECULAR STRUCTURE
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RATS
RECOMBINANT DNA
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES