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Gastrointestinal syndrome and mucosal clonogenic cells: relationships between target cell sensitivities, LD/sub 50/ and cell survival, and their modification by antibiotics

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576169· OSTI ID:5653237
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The sensitivity of the target cells responsible for the gastrointestinal syndrome in mice was deduced from the steepness of the dose-survival curve for mice assessed on Day 7 after irradiation. The D/sub 0/ value was 1.25 +- 0.22 Gy, virtually identical to the value of 1.23 +- 0.08 measured for microcolony-forming cells (clonogens) over about the same range of dose in concurrent experiments. The survival of clonogens was similar when assayed in mice surviving to Days 3, 4, or 5, but clonogenic sensitivity was lower when assessed on Day 7. This was shown at one dose to be due largely to a selection of mice with high colony counts with only a small contribution from crypt budding. The LD/sub 50/ for mice corresponded to a surviving fraction of crypts of about 0.35. An injection of 5 mg streptomycin sulphate ip daily for 5 days after irradition increased the latent period by about 1 day, increased the LD/sub 50/ by about 1.4 Gy, but did not significantly change the survival of clonogens. These studies are the first to test and satisfy the interpretation of a dose-response curve for animal survival in terms of target cell survival, where measurements of both are made over a similar range of dose in concurrent experiments.

Research Organization:
Paterson Labs., Christie Hospital and Holt Radium Inst., Manchester, England
OSTI ID:
5653237
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 96:1; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

551000 -- Physiological Systems
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BEAMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CESIUM 137
CESIUM ISOTOPES
CRYPT CELLS
DATA
DIGESTIVE SYSTEM
DOSES
DRUGS
ELECTROMAGNETIC RADIATION
ELECTRON BEAMS
EXPERIMENTAL DATA
EXTERNAL IRRADIATION
GAMMA RADIATION
GASTROINTESTINAL TRACT
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOTOPES
LEPTON BEAMS
LETHAL DOSES
LETHAL RADIATION DOSE
MAMMALS
MICE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
PARTICLE BEAMS
RADIATION DOSES
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
STREPTOMYCIN
SURVIVAL CURVES
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOTOPES