Influence of anabolic agents on protein synthesis and degradation in muscle cells grown in culture

Journal Article · · Growth; (United States)

OSTI ID:5652770

Roeder, R A; Thorpe, S D; Byers, F M; Schelling, G T; Gunn, J M

Muscle cell culture (L/sub 6/) studies were conducted to determine whether anabolic agents have a direct effect on the muscle cell. The effect of zeranol, testosterone propionate, estradiol benzoate, progesterone, dexamethasone and anabolic agent-dexamethasone combinations on protein synthesis and degradation were measured. Myoblast and myotube cultures were pretreated with 1 ..mu..M compounds for 12, 24 and 48 h before a 6-h synthesis or degradation measuring period. Protein synthesis was determined as cpm of (/sup 3/H) leucine incorporated per mg cell protein. Protein degradation was measured by a pulse-chase procedure using (/sup 3/H) leucine and expressed as the percentage labeled protein degraded in 6 h. Progesterone slightly increased protein synthesis in myoblast cultures. Testosterone propionate had no effect on synthesis. Protein synthesis was decreased by estradiol benzoate in myotube cultures. Protein degradation was not altered appreciably by anabolic agents. Protein synthesis was initially inhibited in myotubes by dexamethasone, but increased in myoblasts and myotubes in the extended incubation time. Dexamethasone also consistently increased protein degradation, but this required several hours to be expressed. Anabolic agents did not interfere with dexamethasone-induced increases in protein synthesis and degradation. The magnitude of response and sensitivity were similar for both the myoblast and the more fully differentiated myotube for all compounds tested. These results indicate that anabolic agents at the 1 ..mu..M level do not have a direct anabolic effect on muscle or alter glucocorticoid-induced catabolic response in muscle.

• Research Organization: Texas A and M Univ., College Station
• OSTI ID: 5652770
• Journal Information: Growth; (United States), Journal Name: Growth; (United States) Vol. 50:4; ISSN GROWA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINO ACIDS
ANABOLISM
ANDROGENS
ANDROSTANES
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CATABOLISM
CELL CULTURES
CORTICOSTEROIDS
DEXAMETHASONE
ESTRADIOL
ESTRANES
ESTROGENS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LEUCINE
METABOLISM
MONOCARBOXYLIC ACIDS
MUSCLES
MYOBLASTS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PREGNANES
PROGESTERONE
PROPIONIC ACID
PROTEINS
STEROID HORMONES
STEROIDS
TESTOSTERONE
TRACER TECHNIQUES
TRITIUM COMPOUNDS