Effects of normal mouse serum on macromolecular synthesis by cultured chick limb myoblasts
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5388831
Previous studies indicate that normal mouse serum exhibits inhibitory effects on embryonic chick skeletal muscle cell differentiation. In addition, following administration of the test serum, large amounts of lipid droplets are evident in the sacroplasm. The present study was undertaken to assess the role of mouse serum on such parameters as cell fusion, a prerequisite for the production of multinucleated myotubes, macromolecular synthesis and the nature of the accumulated lipids produced in response to treatment. The results of this study indicate that mouse serum contains a factor(s) which acts in a dose-response manner to inhibit the fusion of myoblasts into functional multinucleated myotubes which synthesize and organize myofibrillar proteins. DNA synthesis by the serum-treated cells was significantly reduced by 24 hr, prior to the removal of fused myoblasts from the proliferating pool, as seen in the control cultures. Protein synthesis remained linear but somewhat reduced over a 5-hr labelling period with /sup 3/H-leucine following 24 hr of in vitro culture. However, following repeated treatments over several days, the cells showed a significant decrease in protein synthesis when compared to differentiated controls. In addition, there was a significant increase in the content of neutral lipids of treated cells.
- Research Organization:
- Atlanta Univ., GA
- OSTI ID:
- 5388831
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BIRDS
BLOOD SERUM
CARBOXYLIC ACIDS
CELL CULTURES
CELL PROLIFERATION
CHICKENS
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
FOWL
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
LIPIDS
MAMMALS
MICE
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BIRDS
BLOOD SERUM
CARBOXYLIC ACIDS
CELL CULTURES
CELL PROLIFERATION
CHICKENS
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
FOWL
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
LIPIDS
MAMMALS
MICE
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES