Estimate of the probability of diffusional misordering in high-speed DNA sequencing

Journal Article · Thu Oct 07 00:00:00 EDT 1993 · Journal of Physical Chemistry; (United States)

DOI:https://doi.org/10.1021/j100142a002· OSTI ID:5651202

Pratt, L R; Keller, R A ^[1]

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1. Los Alamos National Lab., NM (United States)

The issue of diffusional misordering of sequentially cleaved pairs of nucleic acid bases in high-speed DNA sequencing is studied by extracting the expected fraction of misordered pairs from solutions of diffusion-flow equations. These results are used to determine cutting rates, flow velocities, and distances to detectors that correspond to target fractions of misordered pairs. 4 refs., 2 figs.

• OSTI ID: 5651202
• Journal Information: Journal of Physical Chemistry; (United States), Journal Name: Journal of Physical Chemistry; (United States) Vol. 97:40; ISSN JPCHAX; ISSN 0022-3654
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201* -- Chemical & Physicochemical Properties
99 GENERAL AND MISCELLANEOUS
990200 -- Mathematics & Computers
DIFFUSION
DISPERSIONS
DNA SEQUENCING
MATHEMATICAL MODELS
MIXTURES
NUCLEIC ACIDS
ORDER-DISORDER TRANSFORMATIONS
ORGANIC COMPOUNDS
PHASE TRANSFORMATIONS
PROBABILISTIC ESTIMATION
SOLUTIONS
STRUCTURAL CHEMICAL ANALYSIS