Suppressive effect on polyclonal B-cell activation of a synthetic peptide homologous to a transmembrane component of oncogenic retroviruses
Purified feline leukemia virus, UV light-inactivated feline leukemia virus, and a synthetic peptide (CKS-17) homologous to a well-conserved region of the transmembrane components of several human and animal retroviruses were each studied for their effect on IgG production by feline peripheral blood lymphocytes. Using a reverse hemolytic plaque assay, both the viable virus and the UV-inactivated feline leukemia virus, but not the CKS-17, activated B lymphocytes to secrete IgG. When staphylococcal protein A, a polyclonal B-cell activator, was used to stimulate IgG synthesis by feline lymphocytes, the viable virus, the UV-inactivated virus, and the CKS-17 peptide each strongly suppressed IgG secretion without compromising viability of the lymphocytes. These finding suggest that the immunosuppressive influences of feline leukemia virus on immunoglobulin synthesis may reside in a conserved portion of the envelope glycoprotein that includes the region homologous to CKS-17.
- Research Organization:
- Univ. of South Florida, St. Petersburg
- OSTI ID:
- 5650543
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IMMUNOGLOBULINS
SECRETION
LEUKEMIA VIRUSES
BIOLOGICAL RADIATION EFFECTS
INACTIVATION
PEPTIDES
BIOCHEMISTRY
BIOSYNTHESIS
GLUCOPROTEINS
LYMPHOCYTES
ULTRAVIOLET RADIATION
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CHEMISTRY
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
GLOBULINS
LEUKOCYTES
MATERIALS
MICROORGANISMS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIATION EFFECTS
RADIATIONS
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
VIRUSES
560130* - Radiation Effects on Microorganisms