Biosynthesis and degradation of collagen in X-irradiated mouse lung

Journal Article · Sun Nov 01 04:00:00 EST 1987 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577261· OSTI ID:5650106

Walklin, C M; Freedman, R B; Law, M P

Fibrosis, characterized by accumulation of collagen, is a delayed result of radiation injury in many tissues, including lung. To investigate its development, synthesis and degradation of collagen were measured in lungs of mice after X irradiation of the whole thorax. The ratio of type I (coarse fibered) to type III (meshwork) collagen was also determined. Synthesis of procollagen, measured as the activities of prolyl-4-hydroxylase and protein disulfide isomerase in lung tissue, was increased at 2 months after X-ray doses of 5, 7.5, and 9 Gy. Maximal increases were observed 6 to 7 months after doses of 9 Gy and persisted up to 15 months after exposure. Increases after 5 and 7.5 Gy were more gradual, but by 1 year after irradiation they had reached levels similar to those after 9 Gy. X irradiation had no effect on the degradation of collagen as assessed by collagenase activity in lung. The ratio of type I to type III collagen, analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of collagen-derived cyanogen bromide peptides, was the same in irradiated lungs as in age-matched controls. Therefore, increased synthesis of procollagen, rather than decreased degradation of collagen or changes in collagen type, is an important factor in the accumulation of collagen in irradiated lung.

• Research Organization: MRC Cyclotron Unit, London, England
• OSTI ID: 5650106
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 112:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
BODY AREAS
CHEST
COLLAGEN
DISEASES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
FIBROSIS
HYDROXYLASES
IONIZING RADIATIONS
ISOMERASES
LUNGS
MAMMALS
METABOLISM
MICE
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PATHOLOGICAL CHANGES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
SCLEROPROTEINS
SYNTHESIS
VERTEBRATES
X RADIATION