Isolation of chromosomal mutations in Drosophila melanogaster by the nondisjunction test

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Sov. Genet. (Engl. Transl.); (United States)

OSTI ID:5649908

Chadov, B F; Ivanov, Yu N; Artemova, E V

The females carrying the combination of metacentric autosome 2 with two acrocentrics, F(2L) and F(2R), produce aneuploid egg cells 2/F(2L) and F(2R)(2-F(2L) nondisjunction) as well as egg cells 2/F(2R) and F(2L) (2-F(2R) nondisjunction). The frequency of nondisjunction 2-F(2L) sharply increases in the presence of inversion in the left arm of metacentric 2, and the frequency of nondisjunction 2-F(2R) increases when the right arm of the metacentric carries the inversion. The presence of the rearrangement in the left arm can be detected by the unusually high progeny size in the cross between 2/F(2L);F(2R) females, and C(2L);F(2R)/F(2R) males, and the presence of rearrangement in the right arm of the metacentric is inferred from a large progeny in the cross of similar females with F(2L)/F(2L);C(2R) males. The SPx/sup 2//F(2L);F(2R) daughters were obtained from SPx/sup 2//+ males irradiated with the dose of 3000R. Out of these, 972 daughters were individually crossed with F(2L)/F(2L); C(2R) males. Fifty-two cultures with large progeny size were identified by visual observation. Preparations of polytene chromsomes were made from the larvae. Twenty-six cultures had arrangements in 2 R: eight carried rearrangement In (2R), six In(2LR), ten T(2R; 3) and T(2R; X), and two Tp (2). The method can be used effective to detect chromosomal mutations, especially inversions in large samples.

• Research Organization: Institute of Cytology and Genetics, Novosibirsk, USSR
• OSTI ID: 5649908
• Journal Information: Sov. Genet. (Engl. Transl.); (United States), Journal Name: Sov. Genet. (Engl. Transl.); (United States) Vol. 21:7; ISSN SOGEB
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEUPLOIDY
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CYTOLOGY
DIPTERA
DROSOPHILA
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
FLIES
FRUIT FLIES
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GENETICS
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
IRRADIATION
LARVAE
MUTATIONS
NON-DISJUNCTION
PLOIDY
PROGENY
RADIATION EFFECTS
RADIATIONS