Enzyme induction in rat lung and liver by condensates and fractions from main-stream and side-stream cigarette smoke

Journal Article · Tue Dec 01 04:00:00 EST 1987 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/S0013-9351(87)80239-6· OSTI ID:5649469

Pasquini, R; Sforzolini, G S; Savino, A; Angeli, G; Monarca, S

Aryl hydrocarbon hydroxylase (AHH) and dimethylnitrosamine demethylase (DMND) activities in pulmonary and hepatic tissues of male Sprague-Dawley rats were assayed following pretreatment with known inducers (benzo(a)pyrene, 3-methylcholanthrene, Aroclor 1254, phenobarbital) and with main-stream (MS) and side-stream (SS) cigarette smoke condensates and their related fractions. Biochemical assays by spectrophotofluorimetry (AHH activity) and spetrophotometry (DMND activity) and by a biological assay (Ames test) were performed to detect AHH and DMND induction. Ames test proved to be much less sensitive than the spectrophotofluorimetric analysis for AHH determination. Both main-stream and side-stream cigarette smoke condensates and some fractions, containing water-soluble bases, water-insoluble bases, and polycyclic aromatic hydrocarbons, were found to induce AHH activity in lung and liver, the lung being induced to the greatest extent. The highest levels of AHH inducibility were found for the SS-smoke condensate and related fractions. In particular, the insoluble bases fractions gave the highest induction. On the contrary, pulmonary DMND activity was not affected by pretreatment with the same materials, while hepatic DMND response was only minimally induced by Aroclor and phenobarbital treatment.

• Research Organization: Univ. of Perugia, Italy
• OSTI ID: 5649469
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 44:2; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
ANESTHETICS
ANIMALS
ANTICONVULSANTS
AROMATICS
ARYL 4-MONOOXYGENASE
AZINES
BARBITURATES
BENZOPYRENE
BODY
CARCINOGENESIS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHLORINATED AROMATIC HYDROCARBONS
COLLOIDS
CONDENSED AROMATICS
DIGESTIVE SYSTEM
DISPERSIONS
DRUGS
ENZYME INDUCTION
ENZYMES
GENE REGULATION
GLANDS
HALOGENATED AROMATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXYLASES
HYPNOTICS AND SEDATIVES
LIVER
LUNGS
MAMMALS
METABOLIC ACTIVATION
MUTAGENESIS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PATHOGENESIS
PHENOBARBITAL
POLYCYCLIC AROMATIC HYDROCARBONS
PYRIMIDINES
RATS
RESIDUES
RESPIRATORY SYSTEM
RODENTS
SMOKES
SOLS
TOBACCO SMOKES
TOXICITY
VERTEBRATES