Quantitative Mutation Detection Using Two-Dimensional Electrophoresis
- Argonne National Laboratory (ANL), Argonne, IL (United States)
Two-dimensional electrophoresis (2DE) of proteins has been used in several studies to detect genetic mutations. Mutations are generally observable in 2DE patterns as spots which appear out of context, either in position or amount (quantitative variants). Most studies to this point have screened only for positional variants, as these are detectable by eye and do not require computer analysis. Screening for quantitative variants in addition to the positional ones widens the class of detectable events. We have been using 2DE to detect heritable mutations expressed as quantitative alterations in liver protein expression in inbred mice with the goal of extending these studies to analysis of mutation expression in human populations. The purpose of this paper is to describe the computerized screening techniques which have been developed and to summarize their performance.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 5644294
- Report Number(s):
- ANL/CP--73117; CONF-9107133--1; ON: DE91014015
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTOMATION
BARYONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
BODY
CHEMISTRY
COBALT 60
COBALT ISOTOPES
COLLOIDS
COMPUTER CALCULATIONS
DATA ANALYSIS
DETECTION
DIGESTIVE SYSTEM
DISPERSIONS
ELECTROPHORESIS
ELEMENTARY PARTICLES
FERMIONS
GELS
GLANDS
HADRONS
IMAGE PROCESSING
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIVER
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
MOLECULAR BIOLOGY
MUTATIONS
NEUTRONS
NITROSO COMPOUNDS
NITROSOUREAS
NUCLEI
NUCLEONS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PERFORMANCE TESTING
PROCESSING
PROTEINS
RADIOISOTOPES
RESEARCH PROGRAMS
RODENTS
TESTING
TWO-DIMENSIONAL ELECTROPHORESIS
USES
VERTEBRATES
YEARS LIVING RADIOISOT