Variable effects of soman on macromolecular secretion by ferret trachea

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Fundamental and Applied Toxicology; (USA)

DOI:https://doi.org/10.1016/0272-0590(91)90131-M· OSTI ID:5643281

McBride, R K; Zwierzynski, D J; Stone, K K; Culp, D J; Marin, M G ^[1]

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1. Univ. of Rochester School of Medicine and Dentistry, NY (USA)

The purpose of this study was to examine the effect of the anticholinesterase agent, soman, on macromolecular secretion by ferret trachea, in vitro. We mounted pieces of ferret trachea in Ussing-type chambers. Secreted sulfated macromolecules were radiolabeled by adding 500 microCi of {sup 35}SO{sub 4} to the submucosal medium and incubating for 17 hr. Soman added to the submucosal side produced a concentration-dependent increase in radiolabeled macromolecular release with a maximal secretory response (mean +/- SD) of 202 +/- 125% (n = 8) relative to the basal secretion rate at a concentration of 10{sup {minus} 7} M. The addition of either 10{sup {minus}6} M pralidoxime (acetylcholinesterase reactivator) or 10{sup {minus}6} M atropine blocked the response to 10{sup {minus}7} M soman. At soman concentrations greater than 10{sup {minus}7} M, secretion rate decreased and was not significantly different from basal secretion. Additional experiments utilizing acetylcholine and the acetylcholinesterase inhibitor, physostigmine, suggest that inhibition of secretion by high concentrations of soman may be due to a secondary antagonistic effect of soman on muscarinic receptors.

• OSTI ID: 5643281
• Journal Information: Fundamental and Applied Toxicology; (USA), Journal Name: Fundamental and Applied Toxicology; (USA) Vol. 16:1; ISSN 0272-0590; ISSN FAATD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALKALOIDS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ATROPINE
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYME INHIBITORS
ENZYMES
ESTERS
EVEN-ODD NUCLEI
HEMIACETAL DEHYDROGENASES
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LACTATE DEHYDROGENASE
LIGHT NUCLEI
MAMMALS
MEMBRANE PROTEINS
NEUROREGULATORS
NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
SECRETION
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRACER TECHNIQUES
TRACHEA
VERTEBRATES