Vasoactive intestinal peptide stimulates tracheal submucosal gland secretion in ferret

Journal Article · Fri Jul 01 04:00:00 EDT 1983 · Am. Rev. Respir. Dis.; (United States)

DOI:https://doi.org/10.1164/arrd.1983.128.1.89· OSTI ID:5586656

Peatfield, A C; Barnes, P J; Bratcher, C; Nadel, J A; Davis, B

We studied the effect of vasoactive intestinal peptide (VIP) on the output of 35S-labeled macromolecules from ferret tracheal explants either placed in beakers or suspended in modified Ussing chambers. In Ussing chamber experiments, the radiolabel precursor, sodium (35S)sulfate, and all drugs were placed on the submucosal side of the tissue. Washings were collected at 30-min intervals from the luminal side and were dialyzed to remove unbound 35S, leaving radiolabeled macromolecules. Vasoactive intestinal peptide at 3 X 10(-7) M stimulated bound 35S output by a mean of + 252.6% (n . 14). The VIP response was dose-dependent with a near maximal response and a half maximal response at approximately 10(-6) M and 10(-8), M, respectively. The VIP effect was not inhibited by a mixture of tetrodotoxin, atropine, I-propranolol, and phentolamine. Vasoactive intestinal peptide had no effect on the electrical properties of the of the tissues. We conclude that VIP stimulates output of sulfated-macromolecules from ferret tracheal submucosal glands without stimulating ion transport. Our studies also suggest that VIP acts on submucosal glands via specific VIP receptors. Vasoactive intestinal peptide has been shown to increase intracellular levels of cyclic AMP, and we suggest that this may be the mechanism for its effect on the output of macromolecules. This mechanism may be important in the neural regulation of submucosal gland secretion.

• Research Organization: Cardiovascular Research Institute, University of California, San Francisco
• OSTI ID: 5586656
• Journal Information: Am. Rev. Respir. Dis.; (United States), Journal Name: Am. Rev. Respir. Dis.; (United States) Vol. 128:1; ISSN ARRDA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
INTESTINES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANES
METABOLIC ACTIVATION
MUCOUS MEMBRANES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIOISOTOPES
RESPIRATORY SYSTEM
STIMULATION
SULFUR 35
SULFUR ISOTOPES
TRACHEA
VERTEBRATES