Induction of mouse mammary tumor virus RNA in mammary tumors of BALB/c mice treated with urethane, x-irradiation, and hormones

Journal Article · Sun Jul 01 04:00:00 EDT 1979 · J. Virol.; (United States)

OSTI ID:5642111

Michalides, R; van Deemter, L; Nusse, R; Hageman, P

The involvement of mouse mammary tumor virus (MTV) in the development of mammary tumors of nonviral etiology in BALB/c mice was studied by measuring the levels of MTV RNA, MTV DNA, and MTV proteins in spontaneously arising and hormally, chemically, and/or physically induced mammary tumors of BALB/c females. The following results were obtained: (1) spontaneous mammary tumors contained very low levels of MTV RNA; 4 x 10/sup -6/% of the cytoplasmic RNA was MTV RNA. No MTV proteins could be demonstrated by using sensitive radioimmunoassays for MTV proteins p27 and gp52. (2) Mammary tumors induced by treatments with urethane or x-irradiation alone contained higher levels of MTV RNA; these tumors contained 3- and 19-fold more MTV RNA, respectively, compared with spontaneous mammary tumors. (3) Mammary tumors induced by combined treatment with urethane and x-irradiation expressed high levels of MTV RNA in the mammary tumors; a 1,724-fold increase in MTV RNA content compared with spontaneous mammary tumors was observed. However, very low levels of MTV proteins gp52 and p27 were detected, suggesting some kind of impairment at the translation of MTV RNA. MTV RNA was also induced by this treatment in mammary glands and spleens, but not in the livers of tumor-bearing animals. (4) BALB/c females continuously exposed to prolactin contained high levels of MTV RNA and MTV proteins in stimulated mammary glands and in the hormonally induced mammary tumors. These findings suggest that MTV is not responsible for the maintenance and probably also not for the development of all murine mammary cancers.

• Research Organization: Netherlands Cancer Inst., Amsterdam
• OSTI ID: 5642111
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 31:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CARCINOGENESIS
DIGESTIVE SYSTEM
DISEASES
DNA
ELECTROMAGNETIC RADIATION
ETIOLOGY
EXPERIMENTAL NEOPLASMS
GLANDS
GONADOTROPINS
HORMONES
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LIVER
LTH
MAMMALS
MAMMARY GLANDS
MICE
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASITES
PATHOGENESIS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIATIONS
RADIOASSAY
RADIOIMMUNOASSAY
RNA
RODENTS
SPLEEN
TRACER TECHNIQUES
URETHANE
VERTEBRATES
VIRUSES
X RADIATION