Cell line established starting with a mouse mammary tumor. Effect of the addition of hormones (in French)
Technical Report
·
OSTI ID:4933964
From 7th international conference on mammary tumors; SaintPierre-de- Chartreuse, France (12 Jun 1972). The PS-MT cell line was defined (18th passage); isolated from a pool of mammary tumors of the PS strain of mice, it remained dormant for 6 months and then grew out very slowly. Subcultures were possible only after 19 months. The morphology is epithelial. After storage in liquid nitrogen in a medium containing 5% DMSO, the viability was approximately 80%. It was not possible to disclose the presence of mycoplasmas. With the standard insulincontaining medium, a few C-type particles were observed by electron-microscopic examination. The addition of hydrocortisone or prolactin, or both hormones together, increases slightly the production of C-type particles. If the secretory stimulating activity of hydrocortisone is maintained for one week before the addition of prolactin for another week, a large amount of A and B particles are found, mixed with C-type particles. They are present in large number in the pellets obtained from the tissue culture media. Five mammary tumors within 6 months were obtained in BALB/c females. Thus, the production of A- and B-type particles is hormone-dependent and requires the same sequence of hormones as the production of casein by mammary glands in organ cultures. (auth)
- Research Organization:
- CEA Centre d'Etudes Nucleaires de Grenoble, 38 (France)
- Sponsoring Organization:
- Sponsor not identified
- NSA Number:
- NSA-29-004717
- OSTI ID:
- 4933964
- Report Number(s):
- CEA-CONF--2288; CONF-720691--2
- Country of Publication:
- France
- Language:
- French
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