In vivo formation of beta-oxidized metabolites of leukotriene E4 in the rat

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Prostaglandins; (USA)

DOI:https://doi.org/10.1016/0090-6980(89)90031-2· OSTI ID:5640981

Perrin, P; Zirrolli, J; Stene, D O; Lellouche, J P; Beaucourt, J P; Murphy, R C ^[1]

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1. Univ. of Colorado Health Sciences Center, Denver (USA)

Intraperitoneal administration of ({sup 3}H)-leukotriene E4 in the rat resulted in the appearance of radiolabel in urine and feces. Separation of polar urinary metabolites and chromatographic comparison of synthetic metabolites indicated the in vivo formation of omega-oxidized metabolites of LTE4 with sequential beta-oxidation. Furthermore, the metabolite identified as 16-carboxy-17,18,19,20-tetranor-14,15-dihydro-N-acetyl-LTE4 substantiates the biochemical pathway of beta-oxidation in vivo involving the 2,4-dienoyl CoA reductase as an integral step. These results substantiate beta-oxidation of sulfidopeptide leukotrienes in vivo and these metabolites account for some of the major urinary metabolites of this class of lipid mediator.

• OSTI ID: 5640981
• Journal Information: Prostaglandins; (USA), Journal Name: Prostaglandins; (USA) Vol. 37:1; ISSN PRGLB; ISSN 0090-6980
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BIOLOGICAL WASTES
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
ENZYMES
FECES
HYDROGEN COMPOUNDS
IN VIVO
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MATERIALS
METABOLISM
METABOLITES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
RATS
REDOX REACTIONS
RODENTS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINE
VERTEBRATES
WASTES