Nuclear magnetic resonance studies of amino acids and proteins. Side-chain mobility of methionine in the crystalline amonio acid and in crystallne sperm whale (Physeter catodon) myoglobin
Deuterium (/sup 2/H) nuclear magnetic resonance (NMR) spectra and spin-lattice relaxation times (T/sub 1/) were obtained of L-(epsilon-/sup 2/H/sub 3/)methionine, L-(epsilon-/sup 2/H/sub 3/)methionine in a D,L lattice, and (S-methyl-/sup 2/H/sub 3/)methionine in the crystalline solid state, as a function of temperature, in addition to obtaining /sup 2/H T/sub 1/ and line-width results as a function of temperature on (epsilon-/sup 2/H/sub 3/)methionine-labeled sperm whale (Physeter catodon) myoglobins by using the method of magnetic ordering. Also recorded were /sup 13/C cross-polarization ''magic-angle'' sample-spinning NMR spectra of (epsilon-/sup 13/C)methionine-labeled crystalline cyanoferrimyoglobin (at 37.7 MHz, corresponding to a magnetic field strength of 3.52 T) and of the same protein in aqueous solution. (JMT)
- Research Organization:
- Univ. of Illinois at Urbana-Champaign
- OSTI ID:
- 5639766
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 22:8; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400301 -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AQUATIC ORGANISMS
CARBON 13
CARBON ISOTOPES
CARBOXYLIC ACIDS
DEUTERIUM
DRUGS
EVEN-ODD NUCLEI
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAGNETIC RESONANCE
MAMMALS
METHIONINE
MOLECULAR STRUCTURE
MYOGLOBIN
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RELAXATION
RESONANCE
SPECTRA
SPIN-LATTICE RELAXATION
STABLE ISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES