Gastrin radioimmunoassay

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5639265

McGuigan, J E; Wolfe, M M

The radioimmunoassay for gastrin is based on competition by unlabeled antigen, i.e., gastrin in the form of synthetic human gastrin I heptadecapeptide (SHG) as standard and (or) gastrin unknown with radiolabeled gastrin (/sub 125/I-SHG), for specific binding sites on antibodies to gastrin, produced in experimental animals. The concentrations of antibody and radiolabeled antigen are kept constant, and standard calibration curves are established by including various concentrations of unlabeled gastrin in the incubation tubes. The antigen, gastrin, is added to concentrations in excess of antibody, to ensure competition between /sub 125/I-SHG and gastrin for the available antibody-binding sites. In this system of competitive inhibition, the proportion of antibody-bound /sub 125/I-SHG is inversely related to the quantity of unlabeled gastrin included in the incubation mixture. Unlabeled antigen (gastrin) available for binding by antibody is either the gastrin present in the standards included in the construction of calibration curves or gastrin present in the unknown samples to be measured. As with other radioimmunoassay techniques, radioimmunoassay of gastrin requires: (a) production of antibodies specific for gastrin, (b) availability of a pure form of the peptide for radiolabeling, (c) preparation of a radiolabeled form of gastrin, and (d) an effective method of separating antibody-bound peptide from free.

• Research Organization: Univ. of Florida Coll. of Medicine, Gainesville
• OSTI ID: 5639265
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:2; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BLOOD SERUM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVALUATION
GASTRIN
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MEASURING METHODS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SENSITIVITY
SPECIFICITY
TRACER TECHNIQUES