Yield of chromosomal aberrations as a function of dosage in primary cultures of Macaca mulatta monkey kidneys fixed at different times. [X rays]

Journal Article · Sun Jan 01 04:00:00 EST 1978 · Radiobiology (USSR) (Engl. Transl.); (United States)

OSTI ID:5638332

Machavariani, M G; Dzhemilev, Z A

A study of the correlation between yield of chromosomal aberrations in a primary culture of monkey kidneys in the first mitosis and investigation of elimination of chromosomal aberrations induced by in vitro irradiation in different doses was conducted. These studies were conducted on primary trypsinized cultures of kidneys from adult Macaca mulatta monkeys. They were exposed immediately after inoculation to radiation delivered by an RUM-13 unit, in doses of 25, 50, 100, 200, and 400 R. In the first series of experiments, cells exposed to radiation immediately after plating were fixed on the 3d day to study the dose-effect function within the first mitosis. In the second series, cells also irradiated immediately after inoculation were fixed at the age of 5 days to examine elimination of chromosomal aberrations after delivery of different doses of radiation. In both the control and with irradiation, there was a low incidence of cells with chromatid aberrations in both experimental series. The difference between incidence of chromatic aberrations in the control and after delivery of different doses of radiation was not significant in both series. In the first series, there is a linear increase in number of chromosomal fragments in the range of 25 to 100 R and nonlinear increase, with higher doses. There is an increase in incidence of radiation-induced breaks per unit dose with increase in radiation dosage. In the second series, the incidence of aberrant cells, total number of chromosomal aberrations and breaks increase linearly with dosage, as a result of which there is virtually no change in number of breaks per 1 R. Regardless of the time of occurrence of the first postradiation mitosis, the mean number of breaks per aberrant cell with dicentrics showed virtually no change, which is indicative of identical radiosensitivity of cells exposed to radiation at the G/sub 0/ stage in both experimental series. (ERB)

• Research Organization: Inst. of Experimental Pathology and Therapy, Sukhumi, USSR
• OSTI ID: 5638332
• Journal Information: Radiobiology (USSR) (Engl. Transl.); (United States), Journal Name: Radiobiology (USSR) (Engl. Transl.); (United States) Vol. 18:2; ISSN RADBA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CULTURES
CELL DIVISION
CHROMOSOMAL ABERRATIONS
DATA
DATA FORMS
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
IN VITRO
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
KIDNEYS
MACACUS
MAMMALS
MITOSIS
MONKEYS
MUTATIONS
NUMERICAL DATA
ORGANS
PRIMATES
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
TIME DEPENDENCE
VERTEBRATES
X RADIATION