Correlation among the rates of dimer excision, DNA repair replication, and recovery of human cells from potentially lethal damage induced by ultraviolet radiation
Journal Article
·
· Biophys. J.; (United States)
The kinetics of excision repair in confluent cultures of diploid human fibroblasts after ultraviolet irradiation at varying doses was measured by three different methods: (1) removal of thymine-containing dimers, (2) DNA excision repair synthesis, and (3) biological recovery of cells from the potentially lethal effects of the irradiation. Each method gave similar results and indicated that the excision rate was dependent upon the number of thymine-containing dimers induced (substrate concentration). For example, at a dose of 40 J/m/sup 2/ (0.2% dimerization), the repair rate was 1.6 J/m/sup 2/ per h as determined by a modified method to measure the number of thymine-containing dimers remaining in DNA and 1.65 J/m/sup 2/ as measured by excision repair synthesis. At a dose of 7.5 J/m/sup 2/, the repair rate was 0.5 J/m/sup 2/ per h as measured by biological recovery, and at a dose of 7 J/m/sup 2/, the repair rate was 0.46 J/m/sup 2/ per h as measured by excision repair synthesis.
- Research Organization:
- Michigan State Univ., East Lansing
- OSTI ID:
- 5637994
- Journal Information:
- Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 28:2; ISSN BIOJA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
560400* -- Other Environmental Pollutant Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CONNECTIVE TISSUE CELLS
DIMERS
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
LETHAL DOSES
MAMMALS
MAN
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
SOMATIC CELLS
THYMINE
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES
560400* -- Other Environmental Pollutant Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CONNECTIVE TISSUE CELLS
DIMERS
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
LETHAL DOSES
MAMMALS
MAN
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
SOMATIC CELLS
THYMINE
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES