Deoxyribonucleic acid excision repair in chromatin after ultraviolet irradiation of human fibroblasts in culture

Journal Article · Tue Sep 04 04:00:00 EDT 1979 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00585a019· OSTI ID:5473877

Williams, J I; Fredberg, E C

We have exposed confluent normal human fibroblasts to ultraviolet (UV) fluences of 5, 14, or 40 J/m/sup 2/ and monitored the specific activity of post-uv repair synthesis in chromatin with (/sup 3/H)thymidine pulses. We have shown that under conditions where no semiconservative deoxyribonucleic acid (DNA) synthesis is detectable, the specific activity of repair label one-fifth that in bulk DNA at all three uv fluences. On the other hand, the distribution of thymine-containing pyrimidine dimers in bulk and nuclease-resistant regions measured either immediately after irradiation or at later times showed no significant differences; preferential labeling of linker (nuclease-sensitive) DNA during repair synthesis is thus apparently not due to a predominance of uv-induced photoproducts in linker relative to core particle DNA in the nucleosoome. Pulse and pulse-chase experiments at 14 or 40 J/m/sup 2/ with normal human or repair-deficient xeroderma pigmentosum (XP) cells showed that at most 30% of repair label in all these cells shifts from nuclease-sensitive (linker) DNA to nuclease-resistant (core particle) DNA.

• Research Organization: Stanford Univ. Medical Center, CA
• OSTI ID: 5473877
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 18:18; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CHROMATIN
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
DIMERS
DNA
EARLY RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FIBROBLASTS
GRAPHS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
LABELLED COMPOUNDS
MAMMALS
MAN
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SOMATIC CELLS
THYMIDINE
THYMINE
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES
XP CELLS