DNA binding and adduct formation of 7,12-dimethylbenz(a)-anthracene by rat mammary epithelial cell aggregates in vitro

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Carcinogenesis; (United States)

DOI:https://doi.org/10.1093/carcin/7.1.95· OSTI ID:5631956

Singletary, K W; Milner, J A

Freshly isolated mammary epithelial cell aggregates from female Sprague-Dawley rats metabolized 7,12-dimethylbenz-(a)anthracene (DMBA) to bay-region anti- and syn-dihydrodiolepoxides that bound to deoxyguanosine and deoxyadenosine residues in cellular DNA. After 24 h of incubation 68% of the DMBA (0.4 micrograms/ml) was metabolized and 58% of the extracellular metabolites were water-soluble. DMBA-DNA binding increased rapidly during the initial 24 h of incubation. Formation of the bay-region syn-dihydrodiolepoxide:deoxyadenosine adduct increased linearly throughout the 24 h, whereas formation of deoxyadenosine and deoxyguanosine adducts with the bay-region anti-dihydrodiolepoxide increased rapidly following a delay of 12 h.

• Research Organization: Univ. of Illinois, Urbana
• OSTI ID: 5631956
• Journal Information: Carcinogenesis; (United States), Journal Name: Carcinogenesis; (United States) Vol. 1; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMAL TISSUES
ANIMALS
AROMATICS
BIOCHEMICAL REACTION KINETICS
BODY
CHEMICAL BONDS
CONDENSED AROMATICS
DIMETHYLBENZANTHRACENE
DNA
DNA ADDUCTS
EPITHELIUM
GLANDS
IN VITRO
KINETICS
LABELLED COMPOUNDS
MAMMALS
MAMMARY GLANDS
METABOLISM
METABOLITES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RATS
REACTION KINETICS
RODENTS
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES