Chromatographic and fluorescence spectroscopic studies of individual 7,12-dimethylbenz(a)anthracene--deoxyribonucleoside adducts

Journal Article · Thu Sep 01 04:00:00 EDT 1983 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/4.9.1201· OSTI ID:5427324

Moschel, R C; Pigott, M A; Costantino, N; Dipple, A

Compared with standard Sephadex LH-20 column chromatography, a newly developed high pressure liquid chromatographic separation of hydrocarbon deoxyribonucleoside adducts derived from the DNA of mouse embryo cell cultures exposed to 7,12-dimethylbenz(a)anthracene (DMBA) provides markedly superior resolution. Once resolved, the fluorescence spectroscopic properties of the three major DMBA--DNA adducts indicate that the fluorescence exhibited by adducts derived from a bay region syn dihydrodiol epoxide of DMBA differs subtly from that exhibited by adducts derived from the isomeric anti dihydrodiol epoxide.

• Research Organization: Basic Research Program-LBI, Frederick Cancer Research Facility, MD
• OSTI ID: 5427324
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 4:9; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

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560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMAL CELLS
ANIMALS
AROMATICS
CELL CULTURES
CHEMICAL COMPOSITION
CHROMATOGRAPHY
CONDENSED AROMATICS
DIMETHYLBENZANTHRACENE
DNA ADDUCTS
EMBRYONIC CELLS
EMISSION SPECTROSCOPY
EPOXIDES
FLUORESCENCE SPECTROSCOPY
ION EXCHANGE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLISM
METABOLITES
MICE
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RODENTS
SEPARATION PROCESSES
SPECTROSCOPY
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES