Preparation and characterization of radioactive castanospermine
Journal Article
·
· Anal. Biochem.; (United States)
A procedure for the preparation of tritiated castanospermine is described. The tritiated alkaloid was shown to be chromatographically identical to the native material and exhibited the same inhibitory properties. Radiolabeled castanospermine tightly bound to purified intestinal sucrase. Following gel chromatography, each mole of enzyme was shown to have bound 1 mol of the radioactive alkaloid. Cultured MDCK cells were also shown to take up the labeled castanospermine. This compound should be a useful tool in the investigation of enzymes that are responsible for the processing of glycoprotein oligosaccharides.
- Research Organization:
- Univ. of Texas Health Science Center, San Antonio
- OSTI ID:
- 5630034
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 163:2; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
ANIMALS
BODY
CELL CULTURES
CHEMICAL COMPOSITION
CHEMICAL PREPARATION
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISTRIBUTION
ENZYMES
GASTROINTESTINAL TRACT
GLYCOSYL HYDROLASES
HYDROLASES
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
ANIMALS
BODY
CELL CULTURES
CHEMICAL COMPOSITION
CHEMICAL PREPARATION
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISTRIBUTION
ENZYMES
GASTROINTESTINAL TRACT
GLYCOSYL HYDROLASES
HYDROLASES
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES