Steroid production and estrogen binding in flowers of Gladiolus

Conference · Wed Apr 01 00:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:5629753

Adler, J H; Wolfe, G R; Janik, J R

The bioconversion of /sup 3/H-cholesterol to steroids was examined in excised tissue from the pistils and bracts of Gladiolus. Ovary-ovule and stigma-style tissues produce a compound with chromatographic properties on reverse phase HPLC similar to 17..beta..-estradiol (E/sub 2/). The stigma-style fraction also produced a compound that chromatographed similarly to progesterone. Bracts and the oxidation controls produced no radiolabeled compounds which were chromatographically similar to E/sub 2/. An endogenous E/sub 2/ binding protein was partially characterized from the ovules. The protein binds E/sub 2/, estriol, and diethylstilbesterol whereas testosterone and progesterone do not bind. The total specific binding capacities in the cytosolic and nuclear fractions are 1.6 and 2.2 femtomoles of estradiol per mg of tissue. The dissociation constant is 1.1 x 10/sup -9/ M/sup -1/ for both subcellular fractions. The protein-estradiol complex has a sedimentation coefficient of 4.7 +/- 0.1S. The tissue specific biosynthesis of estrogens and the presence of a steroid binding protein similar to a Type 1 estrogen receptor found in mammals is suggestive of a role for steroids in pistil ontogeny.

• Research Organization: Michigan Technological Univ., Houghton
• OSTI ID: 5629753
• Report Number(s): CONF-8707108-; TRN: 88-001676
• Journal Information: Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FLOWERS
ONTOGENESIS
PROTEINS
BIOSYNTHESIS
STEROIDS
BIOLOGICAL FUNCTIONS
BIOCHEMICAL REACTION KINETICS
LIQUID COLUMN CHROMATOGRAPHY
ORNAMENTAL PLANTS
PHYSIOLOGY
REPRODUCTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CHROMATOGRAPHY
FUNCTIONS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PLANTS
REACTION KINETICS
SEPARATION PROCESSES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques