Glycerolipid biosynthesis in isolated pea root plastids

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5628279

Xue, Lingru; Sparace, S A ^[1]

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1. McGill Univ., Ste Anne de Bellevue, Quebec (Canada)

Plastids have been isolated from germinating pea (Pisum sativum L.) roots by differential centrifugation and purified on Percoll gradients. Marker enzymes (NADPH: cytochrome c reductase, fumarase and fatty acid synthesis) indicate that greater than 50% of the plastids are recovered essentially free from mitochondrial and endoplasmic reticulum contamination. Fatty acids synthesized from ({sup 14}C)acetate by Percoll-purified plastids are primarily 16:0, 16:1 and 18:1. ({sup 14}C)Acetate-labelled fatty acids and ({sup 14}C)glycerol-3-phosphate are both readily incorporated into glycerolipid. Approximately 12% of the total activity for glycerolipid biosynthesis from glycerol-3-phosphate is recovered in the purified plastid fraction. Glycerolipids synthesized from these precursors are primarily TAG, DAG, PE, PG, PC, PI and PA. Acyl-CoA's also accumulate when acetate is the precursor.

• OSTI ID: 5628279
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
ENDOPLASMIC RETICULUM
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
LIPIDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
ORGANOIDS
PISUM
PLANTS
SYNTHESIS
TRACER TECHNIQUES