Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Phospholipase Dζ Enhances Diacylglycerol Flux into Triacylglycerol

Journal Article · · Plant Physiology (Bethesda)
DOI:https://doi.org/10.1104/pp.17.00026· OSTI ID:1422402
 [1];  [2];  [2];  [2];  [2];  [2]
  1. Donald Danforth Plant Science Center, St. Louis, MO (United States); United States Department of Agriculture Agricultural Research Service (USDA-ARS). Plant Genetics Research Unit; Univ. of Missouri, St. Louis, MI (United States). Dept. of Biology; Univ. of Southern Mississippi, Hattiesburg, MS (United States). Dept. of Chemistry and Biochemistry; Donald Danforth Plant Science Center
  2. Donald Danforth Plant Science Center, St. Louis, MO (United States); United States Department of Agriculture Agricultural Research Service (USDA-ARS). Plant Genetics Research Unit; Univ. of Missouri, St. Louis, MI (United States). Dept. of Biology; Univ. of Southern Mississippi, Hattiesburg, MS (United States). Dept. of Chemistry and Biochemistry
+ Show Author Affiliations

Plant seeds are the primary source of triacylglycerols (TAG) for food, feed, fuel, and industrial applications. As TAG is produced from diacylglycerol (DAG), successful engineering strategies to enhance TAG levels have focused on the conversion of DAG to TAG. However, the production of TAG can be limited by flux through the enzymatic reactions that supply DAG. In this study, two Arabidopsis phospholipase Dζ genes (AtPLDζ1 and AtPLDζ2) were coexpressed in Camelina sativa to test whether the conversion of phosphatidylcholine to DAG impacts TAG levels in seeds. The resulting transgenic plants produced 2% to 3% more TAG as a component of total seed biomass and had increased 18:3 and 20:1 fatty acid levels relative to wild type. Increased DAG and decreased PC levels were examined through the kinetics of lipid assembly by [14C]acetate and [14C]glycerol incorporation into glycerolipids. [14C]acetate was rapidly incorporated into TAG in both wild-type and overexpression lines, indicating a significant flux of nascent and elongated acyl-CoAs into the sn-3 position of TAG. Stereochemical analysis revealed that newly synthesized fatty acids were preferentially incorporated into the sn-2 position of PC, but the sn-1 position of de novo DAG and indicated similar rates of nascent acyl groups into the Kennedy pathway and acyl editing. [14C]glycerol studies demonstrated PC-derived DAG is the major source of DAG for TAG synthesis in both tissues. The results emphasize that the interconversions of DAG and PC pools can impact oil production and composition.

Research Organization:
Donald Danforth Plant Science Center, St. Louis, MO (United States)
Sponsoring Organization:
USDOE Advanced Research Projects Agency - Energy (ARPA-E); United States Department of Agriculture Agricultural Research Service (USDA-ARS)
Grant/Contract Number:
AR0000202
OSTI ID:
1422402
Journal Information:
Plant Physiology (Bethesda), Journal Name: Plant Physiology (Bethesda) Journal Issue: 1 Vol. 174; ISSN 0032-0889
Publisher:
American Society of Plant BiologistsCopyright Statement
Country of Publication:
United States
Language:
English

Cited By (6)

Oil body biogenesis and biotechnology in legume seeds journal September 2017
Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil journal January 2018
PLDα1-knockdown soybean seeds display higher unsaturated glycerolipid contents and seed vigor in high temperature and humidity environments journal January 2019
A transferase interactome that may facilitate channeling of polyunsaturated fatty acid moieties from phosphatidylcholine to triacylglycerol journal September 2019
Genome-Wide Analysis of Phospholipase D Gene Family and Profiling of Phospholipids under Abiotic Stresses in Brassica napus journal May 2019
Spatial analysis of lipid metabolites and expressed genes reveals tissue-specific heterogeneity of lipid metabolism in high- and low-oil Brassica napus L. seeds journal June 2018

Similar Records

Glycerolipid biosynthesis in isolated pea root plastids
Conference · Tue May 01 00:00:00 EDT 1990 · Plant Physiology, Supplement; (USA) · OSTI ID:5628279
Tracking synthesis and turnover of triacylglycerol in leaves
Journal Article · Tue Jan 20 23:00:00 EST 2015 · Journal of Experimental Botany · OSTI ID:1343070

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES