Polyamines and flowering in thin layer tobacco tissue cultures. III. Kinetics of uptake and binding of labelled spermidine

Conference · Wed Apr 01 04:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:5625249

Apelbaum, A; Kaur-Sawhney, R; Galston, A W

/sup 14/C-labelled spermidine (Spd) was supplied to thin layer tobacco tissue cultures growing on Murashige-Skoog medium + 1 ..mu..M NAA and kinetin. Such cultures produce only flower buds, which become obvious after about 15 days. Spd is taken up rapidly at a rate sustained throughout the culture period. Considerable binding of Spd to TCA - precipitable material occurs by day 2 of culture; it increases to a maximum on day 4 and declines by day 10, shortly before flowering. About 80% of the TCA - precipitable label remains in the form of Spd, 12% shows up as putrescine and 3% as spermine. Differential centrifugation revealed binding of Spd to various cell organelles. DNase, RNase and proteases were used to liberate Spd from the bound form. In nuclei it appears that 60% of the label is associated with nucleic acids and 40% with the protein fraction. In view of evidence from animals indicating Spd binding to specific proteins during organogenesis, they are investigating the nature of the Spd binding protein(s).

• Research Organization: Yale Univ., New Haven, CT
• OSTI ID: 5625249
• Report Number(s): CONF-8707108-
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AUXINS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL ACCUMULATION
CARBON 14 COMPOUNDS
CENTRIFUGATION
FLOWERS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PLANT GROWTH REGULATORS
PROTEINS
REACTION KINETICS
SEPARATION PROCESSES
SPERMIDINE
TISSUE CULTURES
TOBACCO
TRACER TECHNIQUES