DNA repair patch size measurements with nucleosomal DNA
Journal Article
·
· Carcinogenesis (N.Y.); (United States)
A modified method for measuring DNA repair patch sizes by the bromodeoxyuridine buoyant density shift method is presented. This method involves the digestion of total chromatin by Staphylococcal nuclease to produce small DNA fragments with a very homogeneous size distribution. Using this method to measure the size of repair patches following treatment with u.v.-light or dimethyl sulfate, a calculated incorporation of 40 nucleotides was obtained. This value is identical to previously published patch size estimations obtained by the buoyant density shift method with sonically produced DNA fragments. It is also in close agreement with values recently obtained by the bromodeoxyuridine photolysis method. Taken together, these independent results obtained with a variety of DNA damaging agents suggest that excision repair patch size is independent of the damaging agent.
- Research Organization:
- Department of Biochemistry, University of Western Ontario, London, Canada
- OSTI ID:
- 5622418
- Journal Information:
- Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 4:8; ISSN CRNGD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIMETABOLITES
ASTROCYTOMAS
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CHROMATIN
DISEASES
DMSO
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
GLIOMAS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
MAMMALS
MAN
MICROORGANISMS
NEOPLASMS
NUCLEASES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOSOMES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHODIESTERASES
PRIMATES
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
STAPHYLOCOCCUS
SULFOXIDES
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIMETABOLITES
ASTROCYTOMAS
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CHROMATIN
DISEASES
DMSO
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
GLIOMAS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
MAMMALS
MAN
MICROORGANISMS
NEOPLASMS
NUCLEASES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOSOMES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHODIESTERASES
PRIMATES
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
STAPHYLOCOCCUS
SULFOXIDES
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES