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Title: Role of neutrophilic elastase in ethanol induced injury to the gastric mucosa

Abstract

Intragastric administration of ethanol (at concentrations likely to be encountered by the mucosa during acute intoxication) produces gastritis. Recent studies have implicated neutrophils in the gastric mucosal injury induced by luminal ethanol. The objective of the present study was to assess whether neutrophilic elastase contributes to the ethanol-induced gastric mucosal injury. Sprague-Dawley rats were instrumented for perfusion of the gastric lumen with saline or ethanol. Mucosal injury was quantitated by continuously measuring the blood-to-lumen clearance of {sup 51}Cr-EDTA. The experimental protocol consisted of a 40 minute control period (saline perfusion) followed by three successive 40 minute experimental periods (ethanol perfusion). During the three experimental periods the concentration of ethanol was progressively increased to 10, 20, and 30%. The experiments were performed in untreated animals and in animals pretreated with either Eglin c (an inhibitor of elastase and cathepsin G activity) or L 658 (a specific inhibitor of elastase activity). The effects of ethanol on EDTA clearance (x control) in untreated (n = 9) and L658 treated (n = 5) animals are shown in the Table below. Pretreatment with L 658 significantly attenuated the ethanol-induced increases in EDTA clearance. Pretreatment with Eglin c (n = 6) also provided some protection againstmore » ethanol-induced injury, but not to the extent as that provided by L658. The results of the authors studies suggest that neutrophilic elastase contributes to a gastric mucosal injury induced by luminal perfusion of the stomach with physiologically relevant concentrations of ethanol.« less

Authors:
;  [1]
  1. Louisiana State Univ. Medical Center, Shreveport (United States)
Publication Date:
OSTI Identifier:
5618815
Report Number(s):
CONF-9004153-
Journal ID: ISSN 0892-6638; CODEN: FAJOE
Resource Type:
Conference
Journal Name:
FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
Additional Journal Information:
Journal Volume: 4:3; Conference: 74. annual meeting of the Federation of American Societies for Experimental Biology, Washington, DC (United States), 1-5 Apr 1990; Journal ID: ISSN 0892-6638
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ETHANOL; TOXICITY; PEPTIDE HYDROLASES; BIOLOGICAL EFFECTS; CHROMIUM 51; EDTA; INFLAMMATION; MUCOUS MEMBRANES; NEUTROPHILS; RADIOASSAY; RATS; STOMACH; ALCOHOLS; AMINO ACIDS; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CARBOXYLIC ACIDS; CHELATING AGENTS; CHROMIUM ISOTOPES; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; EVEN-ODD NUCLEI; GASTROINTESTINAL TRACT; HYDROLASES; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; ISOTOPES; LEUKOCYTES; MAMMALS; MATERIALS; MEMBRANES; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PATHOLOGICAL CHANGES; PROTEINS; RADIOISOTOPES; RODENTS; SYMPTOMS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Kvietys, P R, and Carter, P R. Role of neutrophilic elastase in ethanol induced injury to the gastric mucosa. United States: N. p., 1990. Web.
Kvietys, P R, & Carter, P R. Role of neutrophilic elastase in ethanol induced injury to the gastric mucosa. United States.
Kvietys, P R, and Carter, P R. 1990. "Role of neutrophilic elastase in ethanol induced injury to the gastric mucosa". United States.
@article{osti_5618815,
title = {Role of neutrophilic elastase in ethanol induced injury to the gastric mucosa},
author = {Kvietys, P R and Carter, P R},
abstractNote = {Intragastric administration of ethanol (at concentrations likely to be encountered by the mucosa during acute intoxication) produces gastritis. Recent studies have implicated neutrophils in the gastric mucosal injury induced by luminal ethanol. The objective of the present study was to assess whether neutrophilic elastase contributes to the ethanol-induced gastric mucosal injury. Sprague-Dawley rats were instrumented for perfusion of the gastric lumen with saline or ethanol. Mucosal injury was quantitated by continuously measuring the blood-to-lumen clearance of {sup 51}Cr-EDTA. The experimental protocol consisted of a 40 minute control period (saline perfusion) followed by three successive 40 minute experimental periods (ethanol perfusion). During the three experimental periods the concentration of ethanol was progressively increased to 10, 20, and 30%. The experiments were performed in untreated animals and in animals pretreated with either Eglin c (an inhibitor of elastase and cathepsin G activity) or L 658 (a specific inhibitor of elastase activity). The effects of ethanol on EDTA clearance (x control) in untreated (n = 9) and L658 treated (n = 5) animals are shown in the Table below. Pretreatment with L 658 significantly attenuated the ethanol-induced increases in EDTA clearance. Pretreatment with Eglin c (n = 6) also provided some protection against ethanol-induced injury, but not to the extent as that provided by L658. The results of the authors studies suggest that neutrophilic elastase contributes to a gastric mucosal injury induced by luminal perfusion of the stomach with physiologically relevant concentrations of ethanol.},
doi = {},
url = {https://www.osti.gov/biblio/5618815}, journal = {FASEB Journal (Federation of American Societies for Experimental Biology); (United States)},
issn = {0892-6638},
number = ,
volume = 4:3,
place = {United States},
year = {Mon Feb 26 00:00:00 EST 1990},
month = {Mon Feb 26 00:00:00 EST 1990}
}

Conference:
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