O/sub 3/-induced change in bronchial reactivity to methacholine and airway inflammation in humans

Journal Article · Tue Apr 01 04:00:00 EST 1986 · J. Appl. Physiol.; (United States)

OSTI ID:5616640

Seltzer, J; Bigby, B G; Stulbarg, M; Holtzman, M J; Nadel, J A; Ueki, I F; Leikauf, G D; Goetzl, E J; Boushey, H A

The increase in airway responsiveness induced by O/sub 3/ exposure in dogs is associated with airway epithelial inflammation, as evidenced by an increase in the number of neutrophils (polymorphonuclear leukocytes) found in epithelial biopsies and in bronchoalveolar lavage fluid. We investigated in 10 healthy, human subjects whether O/sub 3/-induced hyperresponsiveness was similarly associated with airway inflammation by examining changes in the types of cells recovered in bronchoalveolar lavage fluid obtained after exposure to air or to O/sub 3/ (0.4 or 0.6 ppm). We also measured the concentrations of cyclooxygenase and lipoxygenase metabolites of arachidonic acid in lavage fluid. We measured airway responsiveness to inhaled methacholine aerosol before and after each exposure and performed bronchoalveolar lavage 3 h later. We found more neutrophils in the lavage fluid from O/sub 3/-exposed subjects, especially in those in whom O/sub 3/ exposure produced an increase in airway responsiveness. We also found significant increases in the concentrations of prostaglandins E2, F2 alpha, and thromboxane B2 in lavage fluid from O/sub 3/-exposed subjects. These results show that in human subjects O/sub 3/-induced hyperresponsiveness to methacholine is associated with an influx of neutrophils into the airways and with changes in the levels of some cyclooxygenase metabolites of arachidonic acid.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 5616640
• Journal Information: J. Appl. Physiol.; (United States), Journal Name: J. Appl. Physiol.; (United States) Vol. 4; ISSN JAPYA
• Country of Publication: United States
• Language: English

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560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BRONCHI
CARBOXYLIC ACIDS
CHOLINE
DRUGS
ENZYMES
HYDROXY COMPOUNDS
INFLAMMATION
LAVAGE
LEUKOCYTES
LIPOTROPIC FACTORS
MAMMALS
MAN
MATERIALS
METABOLITES
MONOCARBOXYLIC ACIDS
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
OZONE
PATHOLOGICAL CHANGES
POLLUTION
PRIMATES
PROSTAGLANDINS
QUATERNARY COMPOUNDS
RESPIRATORY SYSTEM
SYMPTOMS
TOXICITY
VERTEBRATES