Interface contributions to peak broadening in CE-ESI-MS

Conference · Sat Jun 01 00:00:00 EDT 1991

OSTI ID:5615181

Udseth, H R; Barinaga, C J; Smith, R D ^[1]; Whitted, W H ^[2]

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1. Pacific Northwest Lab., Richland, WA (USA)
2. Beckman Instruments, Inc., Palo Alto, CA (USA)

The applications of capillary electrophoresis (CE) are expanding, and a number of commercial CE instruments are now available. Combining CE with mass spectroscopy (MS), first done with an electrospray ionization (ESI) interface, yields additional advantages. Other interfaces have been proposed, but CE-ESI-MS offers better sensitivity, reduced background, applicability to higher molecular weight (MW) compounds and a better interface design. Our aim has been to exploit the advantages of automated CE coupled to MS for separation of biological materials. Details of our instrument design are provided. Samples used for these studies were a mixture of myoglobin proteins (MW {approximately}17 kilodaltons) and a tryptic digest of tuna cytochrome c. The results show the ESI-MS interface does not broaden bands, and ion dissociation in the mass spectrometer permits the unambiguous identification of fragments in cases where mass alone is insufficient. 2 refs., 2 figs. (MHB)

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 5615181
• Report Number(s): PNL-SA-19120; CONF-9105181--26; ON: DE91015262
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BIOLOGICAL MATERIALS
CARBOXYLIC ACIDS
CHARGED PARTICLES
CYTOCHROMES
DESIGN
ELECTROPHORESIS
EQUIPMENT
FEASIBILITY STUDIES
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IONIZATION
IONS
LABORATORY EQUIPMENT
MASS SPECTROMETERS
MASS SPECTROSCOPY
MATERIALS
MEASURING INSTRUMENTS
MOLECULAR IONS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
MYOGLOBIN
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PIGMENTS
POLYPEPTIDES
PORPHYRINS
PROTEINS
SEPARATION PROCESSES
SPECTROMETERS
SPECTROSCOPY