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Insulin and epidermal growth factor receptors in rat liver after administration of the hepatocarcinogen 2-acetylaminofluorene: ligand binding and autophosphorylation

Journal Article · · Cancer Res.; (United States)
OSTI ID:5614770
; ; ; ;
The livers of male F344 rats which were fed 0.02% 2-acetylaminofluorene (2-AAF) for two days or more had decreased binding of insulin and epidermal growth factor (EGF) to their hepatic receptors in microsomal and Golgi fractions. Hepatic receptors which were partially purified from carcinogen-fed rats by Triton X-100 solubilization and wheat germ agglutinin affinity column chromatography also had decreased binding activity compared to receptors from normal rats. Scatchard analysis indicated that the decrease in insulin receptor binding was due to decreased receptor number whereas the change in EGF receptor binding was attributed to decreased receptor affinity. Insulin receptor phosphokinase activity was also decreased in 2-AAF-fed rats and correlated with the decrease in receptor binding. EGF receptor phosphokinase activity was unchanged in 2-AAF-fed rats when stimulated with a high concentration (1 microM) of EGF but was decreased when stimulated with low concentrations (0.01-0.1 microM) of EGF. No EGF or insulin competing activity for receptor binding was found using acid-ethanol extracts of 2-AAF-altered liver. These results suggest that 2-AAF causes different alterations in the insulin and EGF receptors of the rat liver.
Research Organization:
City of Hope National Medical Center, Duarte, CA
OSTI ID:
5614770
Journal Information:
Cancer Res.; (United States), Journal Name: Cancer Res.; (United States); ISSN CNREA
Country of Publication:
United States
Language:
English

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Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
CHEMICAL BONDS
CHEMICAL REACTIONS
CONDENSED AROMATICS
DIGESTIVE SYSTEM
FLUORENE
GLANDS
HORMONES
HYDROCARBONS
INSULIN
LIVER
MAMMALS
MEMBRANE PROTEINS
MICROSOMES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PEPTIDE HORMONES
PHOSPHORYLATION
PITUITARY HORMONES
PROTEINS
RATS
RECEPTORS
RODENTS
STH
VERTEBRATES