Enzyme-based fiber optic sensors

Conference · Tue Dec 01 00:00:00 EST 1987

OSTI ID:5612914

Kulp, T J; Camins, I; Angel, S M

Fiber optic chemical sensors capable of detecting glucose and penicillin were developed. Each consists of a polymer membrane that is covalently attached to the tip of a glass optical fiber. The membrane contains the enzyme and a pH-sensitive fluorescent dye (fluorescein). A signal is produced when the enzyme catalyzes the conversion of the analyte (glucose or penicillin) into a product (gluconic or penicilloic acid, respectively) that lowers the microenvironmental pH of the membrane and consequently, lowers the fluorescence intensity of the dye. Each sensor is capable of responding to analyte concentrations in the range of approx.0.1 to 100 mM. The penicillin optrode response time is 40 to 60 s while that for glucose is approx.5 to 12 min. 7 figs.

• Research Organization: Lawrence Livermore National Lab., CA (USA)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5612914
• Report Number(s): UCRL-97907; CONF-880110-19; ON: DE88005674
• Resource Relation: Conference: SPIE O-E Lase '88: optoelectronics and laser applications in science and engineering, Los Angeles, CA, USA, 10 Jan 1988; Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
GLUCOSE
DETECTION
PENICILLIN
ENZYMES
FLUORESCEIN
MEMBRANES
OPTICAL FIBERS
PH VALUE
ALDEHYDES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AROMATICS
CARBOHYDRATES
CARBOXYLIC ACIDS
DRUGS
DYES
FIBERS
HEXOSES
HYDROXY ACIDS
HYDROXY COMPOUNDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
SACCHARIDES
400102* - Chemical & Spectral Procedures