Enzyme-based fiber optic sensors
Fiber optic chemical sensors capable of detecting glucose and penicillin were developed. Each consists of a polymer membrane that is covalently attached to the tip of a glass optical fiber. The membrane contains the enzyme and a pH-sensitive fluorescent dye (fluorescein). A signal is produced when the enzyme catalyzes the conversion of the analyte (glucose or penicillin) into a product (gluconic or penicilloic acid, respectively) that lowers the microenvironmental pH of the membrane and consequently, lowers the fluorescence intensity of the dye. Each sensor is capable of responding to analyte concentrations in the range of approx.0.1 to 100 mM. The penicillin optrode response time is 40 to 60 s while that for glucose is approx.5 to 12 min. 7 figs.
- Research Organization:
- Lawrence Livermore National Lab., CA (USA)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5612914
- Report Number(s):
- UCRL-97907; CONF-880110-19; ON: DE88005674
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400102* -- Chemical & Spectral Procedures
ALDEHYDES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AROMATICS
CARBOHYDRATES
CARBOXYLIC ACIDS
DETECTION
DRUGS
DYES
ENZYMES
FIBERS
FLUORESCEIN
GLUCOSE
HEXOSES
HYDROXY ACIDS
HYDROXY COMPOUNDS
MEMBRANES
MONOSACCHARIDES
OPTICAL FIBERS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PENICILLIN
PH VALUE
PHENOLS
POLYPHENOLS
SACCHARIDES