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In vitro conditions modify immunoassayability of bovine pituitary prolactin and growth hormone: insights into their secretory granule storage forms

Journal Article · · Endocrinology; (United States)
OSTI ID:5612363
The amount of immunoassayable intracellular bovine (b) PRL and GH varies depending on treatment conditions. The present studies were designed to characterize the mechanisms involved and to compare immunoassayability of both hormones under similar conditions. Pituitary homogenate and secretory granule hormones displayed both time- and temperature-dependent increases when incubated at pH 10.5 with reduced glutathione. Changes in immunoassayability seem to reflect conversion from poorly immunoactive tissue hormone oligomers to monomeric hormone. The data indicate that oligomeric bPRL is stabilized primarily by intermolecular disulfide bonds, although it is also susceptible to urea, SDS, and EDTA; granule thiols may also influence the conversion to monomer. The storage form of bGH appears to be stabilized differently. Maneuvers demonstrated in these studies to influence immunoassayability correlate very well with their previously established effects on hormone release and secretion, strengthening the likelihood that a functional link exists between assayability and secretion.
Research Organization:
Univ. of Rochester School of Medicine and Dentistry, NY
OSTI ID:
5612363
Journal Information:
Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 116:4; ISSN ENDOA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
AMINES
ANIMALS
BODY
CATTLE
COMPARATIVE EVALUATIONS
COWS
DOMESTIC ANIMALS
DRUGS
ENDOCRINE GLANDS
GLANDS
GLUTATHIONE
GONADOTROPINS
HORMONES
IN VITRO
ISOTOPE APPLICATIONS
LTH
MAMMALS
MEA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PH VALUE
PITUITARY GLAND
PITUITARY HORMONES
POLYPEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOPROTECTIVE SUBSTANCES
RUMINANTS
SODIUM COMPOUNDS
STH
THIOLS
TRACER TECHNIQUES
VERTEBRATES