Free lung cell phagocytosis and the effect of cigarette smoke exposure

Journal Article · Sun Jun 01 00:00:00 EDT 1980 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148009069643· OSTI ID:5611015

Fogelmark, B; Rylander, R; Sjoestrand, M; Reininghaus, W

We report on a technique for studying phagocytosis in free lung cells with the use of fungal spores. Free lung cells were obtained from a bronchial lavage. They were incubated with fungal spores and the engulfment of these spores was studied at various time intervals and under different conditions. The phagocytosis process was found to occur from relatively stationary macrophages within the first hours after incubation. The number of engulfed spores was proportional to their number in the solution. Addition of serum or surfactant to the medium increased the phagocytosis rate. In hamsters and rats exposed to tobacco smoke under in vivo conditions, a dose-related increase in phagocytosis rate could be demonstrated.

• OSTI ID: 5611015
• Journal Information: Exp. Lung Res.; (United States), Vol. 1:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
RESPIRATORY TRACT CELLS
SENSITIVITY
TOBACCO SMOKES
BIOLOGICAL EFFECTS
FUNGI
HAMSTERS
MACROPHAGES
PHAGOCYTOSIS
RATS
SPORES
AEROSOLS
ANIMAL CELLS
ANIMALS
COLLOIDS
CONNECTIVE TISSUE CELLS
DISPERSIONS
MAMMALS
PHAGOCYTES
PLANTS
RESIDUES
RODENTS
SMOKES
SOLS
SOMATIC CELLS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
551000 - Physiological Systems
550300 - Cytology