Inactivation of bronchial mucous proteinase inhibitor by cigarette smoke and phagocyte-derived oxidants

Journal Article · Fri Aug 01 04:00:00 EDT 1980 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148009065462· OSTI ID:5610418

Carp, H; Janoff, A

Freshly prepared aqueous solutions of cigarette smoke suppressed the elastase inhibitory capacity (EIC) of the acid-stable proteinase inhibitor present in bronchial mucus (BMPi) and human seminal plasma (HUSI-I). Thin-layer gel-immunofiltration analysis of mixtures of smoke-treated BMPi and human leukocyte elastase showed decreased elastase: BMPi complexes, increased uncomplexed BMPi and increased free elastase. Phenolic antioxidants prevented the suppression of the EIC of BMPi or HUSI-I by cigarette smoke. In addition, treatment of BMPi or HUSI-I with chemical oxidants caused a similar suppression of EIC. Furthermore, treatment of BMPi or HUSI-I with the phagocyte-derived oxidizing system, myeloperoxidase + H2O2 + Cl-, suppressed EIC. Finally, the functional activity of BMPi was significantly reduced in tracheal aspirates of human smokers compared to that of nonsmokers. These results support the hypothesis that local inactivation of BMPi in the conducting airways of the lung by inhaled cigarette smoke or by phagocyte-derived oxidants may play a role in the pathogenesis of obstructive lung disease in smokers.

• OSTI ID: 5610418
• Journal Information: Exp. Lung Res.; (United States), Journal Name: Exp. Lung Res.; (United States) Vol. 1:3; ISSN EXLRD
• Country of Publication: United States
• Language: English

Related Subjects

560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
COLLOIDS
DISEASES
DISPERSIONS
ENZYME INHIBITORS
ENZYMES
HYDROLASES
INACTIVATION
LEUKOCYTES
LUNGS
MATERIALS
ORGANS
OXIDIZERS
PATHOLOGY
PEPTIDE HYDROLASES
PHAGOCYTOSIS
RESIDUES
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
SMOKES
SOLS
TOBACCO SMOKES