Radiation inactivation (target size analysis) of the gonadotropin-releasing hormone receptor: evidence for a high molecular weight complex
In the present study we used radiation inactivation (target size analysis) to measure the functional mol wt of the GnRH receptor while it is still a component of the plasma membrane. This technique is based on the observation that an inverse relationship exists between the dose-dependent inactivation of a macromolecule by ionizing radiation and the size of that macromolecule. This method demonstrates a mol wt of 136,346 +/- 8120 for the GnRH receptor. This estimate is approximately twice that obtained (60,000) by photoaffinity labeling with a radioactive GnRH analog followed by electrophoresis under denaturing conditions and, accordingly, presents the possibility that the functional receptor consists of a high mol wt complex in its native state. The present studies indicate that the GnRH receptor is either a single weight class of protein or several closely related weight classes, such as might occur due to protein glycosylation.
- Research Organization:
- Duke Univ. Medical Center, Durham, NC
- OSTI ID:
- 5609010
- Journal Information:
- Endocrinology; (United States), Vol. 116:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL MEMBRANES
BIOLOGICAL RADIATION EFFECTS
HORMONES
RECEPTORS
MOLECULAR WEIGHT
FEMALES
HYPOTHALAMUS
PITUITARY GLAND
RATS
ANIMALS
BIOLOGICAL EFFECTS
BODY
BRAIN
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
ENDOCRINE GLANDS
GLANDS
MAMMALS
MEMBRANES
NERVOUS SYSTEM
ORGANS
RADIATION EFFECTS
RODENTS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)