Comparative developmental and phenotypic properties of altered hepatocyte foci and hepatic tumors in rats initiated neonatally with different doses of diethylnitrosamine and promoted with dietary phenobarbital

Conference · Thu Jan 01 04:00:00 EST 1987

OSTI ID:5606087

Peraino, C; Carnes, B A; Stevens, F J

Studies in this laboratory indicate that members of the diverse spectrum of hepatic lesions appearing in rats given a single neonatal treatment with carcinogen followed by promotion with dietary phenobarbital are developmentally independent. Our more recent experiments provide a more definitive evaluation of the developmental relationships among these lesions by subdividing the altered hepatocyte focus population into foci that were detectable solely by histochemical means and those that also showed morphological aberrations and comparing the developmental and phenotypic properties of the hist(+), morph-) foci, hist(+), morph(+) foci, and hepatic tumors in rats initiated once neonatally with different doses of diethylnitrosamine and promoted with dietary phenobarbital from weaning. Rats were killed at intervals, and their livers were examined for tumors and for the two classes of altered hepatocyte foci. Through application of serial frozen-sectioning techniques (2) and computer-assisted image analysis (3), foci containing between one and seven histochemical markers and/or exhibiting morphologic changes were identified and sized. The same complement of histochemical tests was applied to the hepatic tumors observed in this investigation. 3 refs.

• Research Organization: Argonne National Lab., IL (USA)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5606087
• Report Number(s): CONF-8706206-3; ON: DE88003896
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANESTHETICS
ANIMAL CELLS
ANIMALS
ANTICONVULSANTS
AZINES
BARBITURATES
CARCINOGENESIS
CARCINOGENS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
COMPARATIVE EVALUATIONS
DISEASES
DRUGS
FETUSES
HETEROCYCLIC COMPOUNDS
HYPNOTICS AND SEDATIVES
LIVER CELLS
MAMMALS
NEOPLASMS
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PATHOGENESIS
PHENOBARBITAL
PROMOTERS
PYRIMIDINES
RATS
RESPONSE MODIFYING FACTORS
RODENTS
SOMATIC CELLS
TUMOR PROMOTERS
VERTEBRATES