Identification and molecular cloning of a soluble human granulocyte-macrophage colony-stimulating factor receptor

Journal Article · Sun Sep 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.18.8203· OSTI ID:5604597

Raines, M A; Liu, Lide; Quan, S G; Joe, V; Golde, D W ^[1]; DiPersio, J F ^[2]

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1. Univ. of California, Los Angeles (United States)
2. Univ. of Rochester, NY (United States)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) plays an important role in hematopoiesis and host defense via interaction with specific cell-surface receptors in target tissues. The authors identified a truncated, soluble form of the low-affinity GM-CSF receptor (GMR) in choriocarcinoma cells. Low-affinity GMR cDNAs encoding both the membrane-bound and soluble receptors were obtained by PCR using primers corresponding to the published sequence. Clones encoding the soluble receptor were identical in sequence to the membrane-bound form but contained a 97-nucleotide internal deletion. The amino acid sequence of this deleted cDNA predicts a protein that lacks the 84 C-terminal amino acids of the membrane-bound receptor, including the transmembrane and cytoplasmic domains, and contains 16 different amino acids at its C terminus. The striking similarity between the soluble form of the GMR and other hematopoietin receptors suggests that soluble binding proteins may play an important role in regulating the broad spectrum of biological responses mediated by these cytokines.

• OSTI ID: 5604597
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:18; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
AMINO ACID SEQUENCE
LYMPHOKINES
RECEPTORS
RECOMBINANT DNA
DNA SEQUENCING
COLONY FORMATION
DNA-CLONING
ELECTROPHORESIS
HEMATOPOIETIC SYSTEM
IODINE 125
MACROPHAGES
PHOSPHORUS 32
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CLONING
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
ELECTRON CAPTURE RADIOISOTOPES
HYBRIDIZATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MITOGENS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry