Repression of the interleukin 6 gene promoter by p53 and the retinoblastoma susceptibility gene product

Journal Article · Sun Sep 01 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.17.7605· OSTI ID:5604146

Santhanam, U; Ray, A; Sehgal, P B ^[1]

---

1. Rockefeller Univ., New York, NY (United States)

The aberrant overexpression of interleukin 6 (IL-6) is implicated as an autocrine mechanism in the enhanced proliferation of the neoplastic cell elements in various B- and T-cell malignancies and in some carcinomas and sarcomas; many of these neoplasms have been shown to be associated with a mutated p53 gene. The possibility that wild-type (wt) p53, a nuclear tumor-suppressor protein, but not its transforming mutants might serve to repress IL-6 gene expression was investigated in HeLa cells. The authors transiently cotransfected these cells with constitutive cytomegalovirus (CMV) enhancer/promoter expression plasmids overproducing wt or mutant human or murine p53 and with appropriate chloramphenicol acetyltransferase (CAT) reporter plasmids containing the promoter elements of human IL-6, c-fos, or {beta}-actin genes or of porcine major histocompatibility complex (MHC) class I gene in pN-38 to evaluate the effect of the various p53 species on these promoters. These observations identify transcriptional repression as a property of p53 and suggest that p53 and RB may be involved as transcriptional repressors in modulating IL-6 gene expression during cellular differentiation and oncogenesis.

• OSTI ID: 5604146
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:17; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACTIN
ANIMAL CELLS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARCINOGENESIS
CELL DIFFERENTIATION
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GENE REGULATION
GENE REPRESSORS
GROWTH FACTORS
HELA CELLS
HISTOCOMPATIBILITY COMPLEX
ISOTOPES
LIGHT NUCLEI
LYMPHOKINES
MITOGENS
MYCELIUM
NUCLEI
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PATHOGENESIS
PLANT TISSUES
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
TISSUES
TRANSCRIPTION
TUMOR CELLS