Fluorescence imaging of single molecules and photosynthetic membranes with two-photon excitation
Conference
·
OSTI ID:560345
- Pacific Northwest National Lab., Richland, WA (United States)
We report the imaging of single-molecule fluorescence induced by two-photon excitation in ambient conditions. Using an inverted fluorescence microscope, we obtained the two-photon images of different single fluorophores (Rhodamine B, Sulforhodamine 101, Coumarin 535 on poly-methyl methacrylate films) and biological membrane fragments by Faster scanning the sample with respect to a diffraction limited focus of a mode-locked Ti: sapphire laser beam. The signal to background ratio was as high as 50:1 and the full width at half maximum (250nm) of a single-molecule peak was significantly shorter than that for one photon excitation. With its high sensitivity and simplicity, the two-photon experiment offers a valuable approach for spectroscopic studies on individual immobilized molecules.
- OSTI ID:
- 560345
- Report Number(s):
- CONF-970443--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Detection and lifetime measurement of single molecules in flowing sample streams by laser-induced fluorescence
Simultaneous time and frequency resolved fluorescence microscopy of single molecules.
Polarization spectroscopy of single fluorescent molecules
Journal Article
·
Mon Apr 26 00:00:00 EDT 1993
· Applied Physics Letters; (United States)
·
OSTI ID:6889130
Simultaneous time and frequency resolved fluorescence microscopy of single molecules.
Technical Report
·
Fri Dec 31 23:00:00 EST 2004
·
OSTI ID:921607
Polarization spectroscopy of single fluorescent molecules
Journal Article
·
Thu Aug 19 00:00:00 EDT 1999
· Journal of Physical Chemistry B: Materials, Surfaces, Interfaces, amp Biophysical
·
OSTI ID:691302