Detection and lifetime measurement of single molecules in flowing sample streams by laser-induced fluorescence

Journal Article · Mon Apr 26 00:00:00 EDT 1993 · Applied Physics Letters; (United States)

DOI:https://doi.org/10.1063/1.109495· OSTI ID:6889130

Wilkerson, Jr, C W; Goodwin, P M; Ambrose, W P; Martin, J C; Keller, R A ^[1]

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1. Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States)

The detection and measurement of fluorescence lifetimes of single Rhodamine-110 molecules in a flowing, aqueous sample stream is described. Time-correlated single-photon counting, used in combination with mode-locked picosecond pulsed excitation, allows the detection of single fluorescent molecules in the presence of significant solvent Raman and Rayleigh backgrounds. The fluorescence lifetime of a detected molecule is estimated from the record of arrival times (relative to the excitation pulse) of photons detected during the molecule's passage through the [similar to]1 pL excitation volume.

• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 6889130
• Journal Information: Applied Physics Letters; (United States), Journal Name: Applied Physics Letters; (United States) Vol. 62:17; ISSN APPLAB; ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400500* -- Photochemistry
665100 -- Nuclear Techniques in Condensed Matter Physics -- (1992-)
75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
AMINES
AQUEOUS SOLUTIONS
CARBOXYLIC ACIDS
DISPERSIONS
DYES
DYNAMICS
ELECTROMAGNETIC RADIATION
FLUID FLOW
FLUORESCENCE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LASER RADIATION
LIFETIME
LUMINESCENCE
MEASURING METHODS
MECHANICS
MIXTURES
MOLECULES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RADIATIONS
REAGENTS
RHODAMINES
SOLUTIONS