T lymphocytes from irradiation chimeras repopulated with 13-day fetal liver cells recognize antigens only in association with self-MHC products

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Journal of Molecular and Cellular Immunology; (USA)

OSTI ID:5601590

Nisbet-Brown, E; Diener, E ^[1]

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1. Univ. of Alberta, Edmonton (Canada)

The restriction specificities of maturing thymocytes are determined by the Class II MHC antigens expressed by non-lymphoid thymic tissues. The proliferative response of mature T lymphocytes to antigen-presenting cells (APC) and antigen requires that the APC express the same MHC antigens as the thymus in which the T cells differentiated. Thus, in the two-way bone marrow chimera (A + B----(A x B)F1), T lymphocyte populations of A and B haplotypes have each acquired the potential to recognize antigens associated with either parental haplotype. In spite of the large body of work on MHC restriction, we still do not have a clear understanding of the mechanisms which impose self restriction. The chimeric model systems used previously to study MHC restriction have used adult bone marrow cells as the source of lymphoid precursors. During normal ontogeny, T cells are derived from precursors in the fetal liver and we felt that a direct comparison of T cells from fetal liver and bone marrow-repopulated animals would shed light on the development of MHC restriction specificities during T cell ontogeny in the thymus or prethymically. We found that parental T lymphocyte populations isolated from two-way fetal liver chimeras cooperated only with syngeneic APC, while those from bone marrow chimeras cooperated with APC of either parental haplotype. This suggests that fetal liver and bone marrow may not be equivalent sources of stem cells. Our results may be due to fundamental differences between thymocyte precursors in fetal liver and bone marrow, including the time course of their expression of T cell receptor gene products.

• OSTI ID: 5601590
• Journal Information: Journal of Molecular and Cellular Immunology; (USA), Journal Name: Journal of Molecular and Cellular Immunology; (USA) Vol. 2:4; ISSN 0724-6803; ISSN JMCID
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CELL PROLIFERATION
CHIMERAS
CONNECTIVE TISSUE CELLS
GENE REGULATION
HEMATOPOIETIC SYSTEM
LEUKOCYTES
LIVER CELLS
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MOSAICISM
ONTOGENESIS
ORGANS
PRECURSOR
RADIATION CHIMERAS
RODENTS
SOMATIC CELLS
STEM CELLS
THYMOCYTES
THYMUS
TISSUES
VERTEBRATES