Involvement of second messengers in regulation of the low-density lipoprotein receptor gene
Journal Article
·
· Molecular and Cellular Biology; (USA)
- Leuven Univ. (Belgium). ECHEM Labs.
- Washington Univ., Seattle, WA (USA). Dept. of Medicine
Transcription of the low-density lipoprotein receptor (LDL-R) gene in the human monocytic leukemic cell line THP-1 and in the human hepatocarcinoma cell line Hep-G2 is regulated by second messengers of the diacylglycerol-protein kinase C (DAG-PKC), inositol 1,4,5-triphosphate-Ca/sup 2+/, and cyclic AMP pathways. Exogeneous phospholipase C (which releases DAG and inositol 1,4,5-triphosphate), PKC activators (phorbol esters and DAG), Ca/sup 2+/ ionophores, and a cyclic AMP analog all transiently induced accumulation of LDL-R mRNA. The effects of these three signal-transducing pathways were to a large extend additive. Furthermore, PKC stimulation effected an increase in LDL binding, which suggested that the increase in LDL-R mRNA resulted in an increase in functional cell surface receptor activity. These results suggest that uptake of cholesterol by these cells is under control of both intracellular cholesterol levels and external signals.
- OSTI ID:
- 5601493
- Journal Information:
- Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 9:6; ISSN MCEBD; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
Similar Records
Formation and actions of calcium-mobilizing messenger, inositol 1,4,5-trisphosphate
Overexpression of protein kinase C. beta. 1 enhances phospholipase D activity and diacylglycerol formation in phorbol ester-stimulated rat fibroblasts
Regulation of cholesterol esterification by protein kinase C
Journal Article
·
Sat Jan 31 23:00:00 EST 1987
· Am. J. Physiol.; (United States)
·
OSTI ID:6285084
Overexpression of protein kinase C. beta. 1 enhances phospholipase D activity and diacylglycerol formation in phorbol ester-stimulated rat fibroblasts
Journal Article
·
Mon Jan 14 23:00:00 EST 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5030689
Regulation of cholesterol esterification by protein kinase C
Conference
·
Tue Mar 04 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6962349
Related Subjects
550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
CELL CULTURES
CHOLESTEROL
DISEASES
ENZYME ACTIVITY
ENZYMES
FUNCTIONS
GENES
HEMIC DISEASES
HYDROXY COMPOUNDS
IMMUNE SYSTEM DISEASES
KINETICS
LEUKEMIA
LIPASE
LIPIDS
LIPOPROTEINS
MEMBRANE PROTEINS
MESSENGER-RNA
METABOLISM
NEOPLASMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
REACTION KINETICS
RECEPTORS
RNA
STEROIDS
STEROLS
TRANSCRIPTION
TRANSFERASES
TUMOR CELLS
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
CELL CULTURES
CHOLESTEROL
DISEASES
ENZYME ACTIVITY
ENZYMES
FUNCTIONS
GENES
HEMIC DISEASES
HYDROXY COMPOUNDS
IMMUNE SYSTEM DISEASES
KINETICS
LEUKEMIA
LIPASE
LIPIDS
LIPOPROTEINS
MEMBRANE PROTEINS
MESSENGER-RNA
METABOLISM
NEOPLASMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
REACTION KINETICS
RECEPTORS
RNA
STEROIDS
STEROLS
TRANSCRIPTION
TRANSFERASES
TUMOR CELLS